Table 2.

Primers and Taqman probes used in the analysis.

primer/probefinal concentration (nM)sequence (5′–3′)amplicon size (bp)
IS1081_F900GCACTCCATCTACGACCAG
IS1081_Probe3006FAM-ATTGGGCAACAACTGATTCGGCGTCG-TAMRA84
IS1081_R900GGGAGTTTGTCGGTCAGAG
rpoB2_F300CAACGTCGAGGTGCTATCG
rpob2_Probe300FAM-TCGCCGCACCGTCACT-NFQMGB70
rpoB2_R300CTCCAGGTCCTCGTCCTCA